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SUMMARY:Plant Science Colloquium with Prof. Erich Glawischnig
DTSTART;TZID=Europe/Berlin:20260123T121500
DTSTAMP:20260527T114252Z
UID:580e024dbb24413d96de15b69659b570@www.inres.uni-bonn.de
CREATED:20240522T123141Z
DESCRIPTION:The expansion of gene families via duplication is a characteri
 stic feature of plant genomes and presents a major challenge for assigning
  biological functions using single-gene knockouts. Here\, we present a str
 ategy for analyzing the biological functions of highly expanded enzyme fam
 ilies in A. thaliana\, using the cytochrome P450 subfamily 71B as an examp
 le. This subfamily contains 32 expressed genes\, and only a few are functi
 onally characterized\, such as CYP71B15 (PAD3)\, an essential component of
  a camalexin biosynthetic complex. Aiming for generation of a complete 32x
  knockout\, we have deleted four CYP71B clusters (up to 83 kb in size) usi
 ng CRISPR/Cas9-based gene editing. Metabolite compositions are being syste
 matically analyzed via UHPLC/Q-TOF-MS suggesting functions contributing to
  defense and/or communication. Here\, a cyp71b11- cyp71b13 deletion mutant
  is impaired in the formation of hydroxycamalexin glycosides\, which resul
 ts in enhanced camalexin autotoxicity.
LAST-MODIFIED:20260112T070905Z
URL:https://www.inres.uni-bonn.de/en/about/events/pflanzenwissenschaftlich
 es-kolloquium-glawischnig
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