02. May 2019


Osthoff, Baldauf and Hochholdinger published a research article in BMC Genomics on transcriptomic adaptions in young barley roots subjected water deficit and salt stress.

Fig. 3 a
Fig. 3 a - Overview of differentially expressed genes (DEGs) between control and stress-treated samples. a Volcano plots depict DEGs for each treatment-by-time combination. Up-regulated DEGs are indicated by blue dots, down-regulated DEGs are indicated by red dots. Total number of DEGs are shown in the upper left and right corner for significant up- and down-regulated DEGs. DEGs that do not exceed the threshold of |log2FC| >1 and FDR ≤1% are depicted in grey. © Alina Osthoff/ University of Bonn
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Water deficit and soil salinity substantially influence plant growth and productivity. When occurring individually, plants often exhibit reduced growth resulting in yield losses. The simultaneous occurrence of these stresses enhances their negative effects. Unraveling the molecular mechanisms of combined abiotic stress responses is essential to secure crop productivity under unfavorable environmental conditions.This study examines the effects of water deficit, salinity and a combination of both on growth and transcriptome plasticity of barley seminal roots by RNA-Seq. Exposure to water deficit and combined stress for more than 4 days significantly reduced total seminal root length. Transcriptome sequencing demonstrated that 60 to 80% of stresstype-specific gene expression responses observed 6 h after treatment were also present after 24 h of stress application. However, after 24 h of stress application, hundreds of additional genes were stress-regulated compared to the short 6 htreatment. Combined salt and water deficit stress application results in a unique transcriptomic response that cannotbe predicted from individual stress responses. Enrichment analyses of gene ontology terms revealed stress type-specificadjustments of gene expression. Further, global reprogramming mediated by transcription factors and consistent over-representation of basic helix-loop-helix (bHLH) transcription factors, heat shock factors (HSF) and ethylene responsefactors (ERF) was observed. This study reveals the complex transcriptomic responses regulating the perception and signaling ofmultiple abiotic stresses in barley.

You can find more here: https://doi.org/10.1186/s12864-019-5634-0

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